Human Neuropeptide W-23/L8 ELISA Kit

Sample

blood, plasma, tissues, CSF

Species Reactivity

Human

Detection Method

cELISA

Intended Use

This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay.

Contents of Kit

1. 20× assay buffer concentrate (50ml)
2. 96 well immunoplate (1 plate)
3. Acetate plate sealer (APS), (3 pieces)
4. Primary antibody (1 vial).
5. Standard peptide (1 vial).
6. Biotinylated peptide (1 vial).
7. Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
8. Positive control (2 vials).
9. Substrate solution (TMB) (12ml)
10. 2N HCl(15ml)
11. Assay diagram (1 sheet).
12. General protocol (1 book).
Note: The kit should be cquilibrated to room temperature (20-23°C) before opening any vials and starting the assay. It is highly recommended that the solutions be used as soon as possible after rehydration, Recommended blood collection protocol is provided on Specimen Collection And Preparation. Each kit contains sufficient reagents for 96 wells and is capable of assaying 40 duplicate samples.

Storage

1. Store the kit at 4°C upon receipt
2. It is highly recommended that solutions be used as soon as possible after rehydration.
3. Store 1× assay buffer at 4°C.
4. Remove any unneeded strips from antibody-coated plate, reseal them in zip-lock foil and keep at 4°C.
5. Keep rehydrated solution of Standard, Biotinylated peptide, Antibody and SA-HRP at 4°C.

Precision

Intra-assay variation: <10%
Inter-assay variation: <15%

Detection Range

0-100ng/ml

Sensitivity

Minimum Detectable Concentration = 0.20ng/ml