Human Anti-Human TCC C5b-9 Monoclonal antibody, clone aE11 [PE]

Antibody Isotype

IgG2a

Clone

aE11

Species Reactivity

Human; Horse; Pig

Conjugate

Application Notes

Suitable for use in FC, IHC, IF.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

General Notes

Monoclonal antibody aE11 reacts with a C9 neoantigen of the terminal complement complex (TCC). The three distinct activation pathways of complement converge with the formation of a C5 convertase. The cleavage of C5 by this convertase initiates the lytic or terminal pathway. In contrast to the activation pathways, which require enzymatic cleavage for activation, the terminal pathway relies on conformational changes induced by binding. Binding of C6 facilitates binding of C7 which alters the conformation of the complex. After binding of C8, a variable number of C9 molecules associate with the C5b678 complex, which is also termed the terminal complement complex (TCC). The formation of TCC causes lysis of cells or can trigger a variety of cellular metabolic pathways resulting in the synthesis and release of inflammatory mediators. The TCC contains neoantigens that are absent from the individual native components. C9 neoantigens are present both in the membrane-bound (MAC) and the fluid-phase (SC5b-9) complex. TCC is present in normal human plasma and increased in patients with complement activation.
DMAB-CLS26034P is suitable for use in FC, IHC, IF.

Alternative Names

Terminal complement complex; MAC complex; complement membrane attack complex; sC5b-9 complex