HiT II-Ab ELISA Kit

Sample

plasma, blood

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The Kit is a solid phase enzyme immunoassay for the quantitative and qualitative detection of IgG antibodies that cause heparin induced thrombocytopenia type II.

Contents of Kit

1. 5X Sample Buffer 1 vial, 20 mL - 5X concentrated
2. 50X Wash Buffer 1 vial, 20 mL - 50X concentrated
3. Negative Control 1 vial, 1.5 mL
4. Positive Control 1 vial, 1.5 mL
5. Cut-off Calibrator 1 vial, 1.5 mL
6. Calibrators 6 vials, 1.5 mL each 0, 3, 10, 30, 100, 300 U/ml
7. Conjugates 1 vial, 15 mL
8. TMB Substrate 1 vial, 15 mL
9. Stop Solution 1 vial, 15 mL
10. Microtiter plate 12 x 8 well strips

Storage

Store all reagents and the microplate at 2-8°C/35-46°F, in their original containers. Once prepared, reconstituted solutions are stable for 1 month at 4°C/39°F, at least. Reagents and the microplate shall be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly. For more detailed information, please download the following document on our website.

Detection Range

0-300 U/mL

Sensitivity

1.0 U/mL