Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Hexokinase (HK) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 20 mL x 1 | 4°C |
| Substrate | Powder x 1 | -20°C |
| Enzyme | Powder x 1 | -20°C |
| Standard | Powder x 1 | -20°C |
| Positive Control | Powder x 1 | -20°C |
| Instruction Manual | 1 | |
Detection Range
4 umol/L - 400 umol/L
General Description
Hexokinases, which catalyze the ATP-dependent phosphorylation of aldo- and keto-hexoses to hexose-6-phosphate, catalyze the first step in several important biochemical pathways. In mammalian tissues, glucose is the predominant substrate for hexokinases. There are four isozymes of hexokinase in mammalian tissue, (HK-I, -II, -III, and -IV), which differ in their kinetic and regulatory properties as well as tissue distribution and cofactor use. The hexokinases catalyze the first step in most of the relevant glucose metabolism pathways. Alterations in hexokinase activity are associated with multiple disorders such as X-linked muscular dystrophy, hemolytic anemia, and cancer. Hexokinase Microplate Assay Kit provides a simple and direct procedure for measuring hexokinase activity in a variety of samples. Hexokinase activity is determined by a coupled enzyme assay, in which glucose is converted to glucose-6-phosphate by hexokinase, which is oxidized by glucose-6- phosphate dehydrogenase to form NADPH. The resulting NADPH reduces a colorless probe resulting in a colorimetric (340 nm) product proportional to the hexokinase activity present.
Citations
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