Detection and quantification of Hemoglobin (Hb) content in serum samples.
Contents of Kit
Component
Quantity
Storage
96-Well Microplate
1
Dye Reagent A
Powder x 1
4°C
Dye Reagent A Diluent
1 mL x 1
4°C
Dye Reagent B
4 mL x 1
4°C
Reaction Buffer
10 mL x 1
4°C
Standard
Powder x 1
4°C
Instruction Manual
1
Storage
2 - 8°C
Detection Range
1 μg/mL - 100 μg/mL
General Description
Hemoglobin, also spelled haemoglobin and abbreviated Hb, is the iron- containing oxygen-transport metalloprotein in the red blood cells of blood in vertebrates and other animals. In mammals, the protein makes up about 97% of the red cell's dry content, and around 35% of the total content (including water). Hemoglobin transports oxygen from the lungs or gills to the rest of the body, such as to the muscles, where it releases its load of oxygen. Hemoglobin also has a variety of other gas-transport and effect- modulation duties, which vary from species to species, and may be quite diverse in invertebrates. The Hemoglobin can react with O-tolidine. The products can be measured at a colorimetric readout at 435 nm.
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