Detection and quantification of Hemoglobin A1c (HbA1c) content in whole blood and other biological fluids samples.
Contents of Kit
Component
Quantity
Storage
96-Well Microplate
1
Hemolytic Agent
10 mL x 1
4°C
Reaction Buffer
10 mL x 1
4°C
Enzyme I
Powder x 1
-20°C, keep in dark
Enzyme II
Powder x 1
-20°C, keep in dark
Dye Reagent
Powder x 1
-20°C, keep in dark
Standard
Powder x 1
4°C
Plate Sealer
3
Instruction Manual
1
Storage
2 - 8°C
Detection Range
0.1 mmol/L - 5 mmol/L
General Description
Creatine is present in vertebrates and helps to supply energy to muscle. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological and neurodegenerative diseases. Hemoglobin A1c Microplate Assay Kit provides an accurate, convenient measure of Hemoglobin A1c concentration in biological fluids such as whole blood and other biological fluids. In the assay, the denatured whole blood sample is decomposed by protease to amino acids, including valine on the glycated hemoglobin β chain. The fructovaline oxidase reacts with glycated valine and produces H2O2, which is coupled to the corresponding chromogen. The concentration can be measured at 570 nm.
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