Sample
Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of GAPDH activity in serum, plasma, tissue extracts, cell lysate, cell culture media, other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 10 mL x 1 | 4°C |
| Substrate | 30 μL x 1 | -20°C |
| Coenzyme | Powder x 1 | -20°C |
| Dye Reagent A | Powder x 1 | 4°C |
| Dye Reagent B | 1 mL x 1 | 4°C |
| Standard | Powder x 1 | -20°C |
| Positive Control | Powder x 1 | -20°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
4 umol/L - 400 umol/L
General Description
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3- Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. GAPDH Activity Microplate Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 492 nm.
Citations
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