Sample
Serum, Plasma, Urine, Saliva, Milk, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Free Fatty Acid (FFA) content in serum, plasma, urine, saliva, milk, cell cultures, and other biological fluid samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Enzyme | 16 mL x 1 | 4°C |
| Dye Reagent | 4 mL x 1 | 4°C |
| Standard (1mmol/L) | 200 μL x 1 | 4°C |
| Instruction Manual | 1 | |
Detection Range
0.01 mmol/L - 1 mmol/L
General Description
Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome. Free Fatty Acid Microplate Assay Kit is a sensitive assay for determining Free Fatty Acid concentration in various samples. Fatty acids are converted to their CoA derivatives, which are subsequently oxidized by the enzyme mix to yield an intermediate. Formed intermediate then reacts with the Free Fatty acid probe to generate color which can be read by a spectrophotometer at OD 550 nm.
Citations
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COA
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