Human Flt-3L ELISA kit

Name: Human Flt-3L ELISA kit
SKU: DEIA5396
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, and cell culture supernatants

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The Human Flt-3L ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Flt-3L in serum, plasma, and cell culture supernatants.

Contents of Kit

1. Flt-3 L Microplate (Item A): 96 wells (12 strips × 8 wells) coated with anti-Human Flt-3 L. Store at 4°C for 1 month. (Return unused wells to the pouch containing desiccant pack, reseal along entire edge.)
2. Wash Buffer Concentrate (20×) (Item B): 25 ml of 20× concentrated solution. Store at 4°C for 1 month.
3. Standard Protein (Item C): 2 vials of Human Flt-3 L. 1 vial is enough to run each standard in duplicate. Store at -80°C for 1 week.
4. Detection Antibody Flt-3 L (Item F): 2 vials of biotinylated anti-Human Flt-3 L. Each vial is enough to assay half the microplate. Store at 4°C for 5 days.
5. HRP-Streptavidin Concentrate (Item G): 200 μl 600× concentrated HRP-conjugated streptavidin. Do not store and reuse.
6. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3,5,5'-tetramethylbenzidine (TMB) in buffer solution.
7. Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.
8. Assay Diluent A (Item D): 30 ml of diluent buffer, 0.09% sodium azide as preservative.
9. Assay Diluent B (Item E): 15 ml of 5× concentrated buffer. Store at 4°C for 1 month.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see "Reagents And Materials Provided".

Precision

Intra-Assay CV%: <10%
Inter-Assay CV%: <12%

Detection Range

1.5 pg/ml - 400 pg/ml

Sensitivity

The minimum detectable dose of Human Flt-3 L was determined to be 1.5 pg/ml. Minimum detectable dose is defined as the analyte concentration resulting in an absorbance that is 2 standard deviations higher than that of the blank (diluent buffer).

Standard Curve

These standard curves are for demonstration only. A standard curve must be run with each assay.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Comparison of Gene Expression in Human Embryonic Stem Cells, hESC-Derived Mesenchymal Stem Cells and Human Mesenchymal Stem Cells

STEM CELLS INTERNATIONAL

Authors: Barbet, Romain; Peiffer, Isabelle; Hatzfeld, Antoinette; Charbord, Pierre; Hatzfeld, Jacques A.

Abstract

We present a strategy to identify developmental/differentiation and plasma membrane marker genes of the most primitive human Mesenchymal Stem Cells (hMSCs). Using sensitive and quantitative TaqMan Low Density Arrays (TLDA) methodology, we compared the expression of 381 genes in human Embryonic Stem Cells (hESCs), hESC-derived MSCs (hES-MSCs), and hMSCs. Analysis of differentiation genes indicated that hES-MSCs express the sarcomeric muscle lineage in addition to the classical mesenchymal lineages, suggesting they are more primitive than hMSCs. Transcript analysis of membrane antigens suggests that IL1R1(low), BMPR1B(low), FLT4(low), LRRC32(low), and CD34 may be good candidates for the detection and isolation of the most primitive hMSCs. The expression in hMSCs of cytokine genes, such as IL6, IL8, or FLT3LG, without expression of the corresponding receptor, suggests a role for these cytokines in the paracrine control of stem cell niches. Our database may be shared with other laboratories in order to explore the considerable clinical potential of hES-MSCs, which appear to represent an intermediate developmental stage between hESCs and hMSCs.

Promoter profiling and coexpression data analysis identifies 24 novel genes that are coregulated with AMPA receptor genes, GRIAs

GENOMICS

Authors: Chong, Allen; Zhang, Zhuo; Choi, Kwok Pui; Choudhary, Vidhu; Djamgoz, Mustafa B. A.; Zhang, Guanglan; Bajic, Vladimir B.

Abstract

We identified a set of transcriptional elements that are conserved and overrepresented within the promoters of human, mouse, and rat GRIAs by comparing these promoters against a collection of 10,741 gene promoters. Cells regulate functional groups of genes by coordinating the transcriptional and/or posttranscriptional mRNA levels of interacting genes. As such, it is expected that functional groups of genes share the same transcriptional features within their promoters. We found 47 genes whose promoters contain the same combination of transcriptional elements that are overrepresented within the promoters of the GRIA gene family. Coexpressed genes may be transcriptionally coregulated, which in turn suggests that these genes may play complementary roles within a particular functional context. Using microarray expression data, we found 24 (of the 47) genes that share not only a similar promoter profile with GRIAs but also a well-correlated gene expression profile and, thus, we believe these to be coregulated with GRIAs. (c) 2006 Elsevier Inc. All rights reserved.