FABP3 (Dog) ELISA Kit

Sample

Plasma (EDTA), Serum, Urine.

Species Reactivity

Dog

Detection Method

sELISA

Intended Use

FABP3 (Dog) ELISA Kit is for the quantitative measurement of Dog FABP3.

Contents of Kit

1. Anti H-FABP coated plate (12 × 8-well strips): 96 (8×12) wells
2. H-FABP Stock: Lyophilized
3. HRP Conjugate: 11 mL
4. Diluent: 50 mL
5. 20× Wash Solution: 50 mL
6. TMB: 11 mL
7. Stop Solution: 11 mL

Storage

Store the lyophilized stock at or below -20°C. The remainder of the kit should be stored at 2-8°C and the microtiter plate should be kept in a sealed bag with desiccant.

General Description

Fatty acid-binding proteins are ~15 kDa cytoplasmic proteins involved in fatty acid transport and metabolism. The isoform expressed in heart (H-FABP) is highly abundant, representing 10-20 mol % of total cytoplasmic protein. H-FABP is released into blood after cardiac injury and provides a sensitive biomarker of myocardial necrosis in several species1-5 . H-FABP is expressed in both cardiac and skeletal muscle. When used as a cardiac biomarker, it is therefore important to rule out skeletal muscle injury. In the absence of cardiac injury, H-FABP can also be used as a biomarker of skeletal muscle damage. As shown in the figure below, we found that H-FABP levels peaked two hours after cardia ischemia-reperfusion in dogs.

Standard Curve

A typical standard curve is shown below. This is for illustration only. A standard curve must be generated for each experiment.