Detection and quantification of Dehydroascorbic Acid (DHA) content in tissue extracts, cell lysate samples.
Contents of Kit
Component
Quantity
Storage
96-Well UV Microplate
1
Assay Buffer
30 mL x 4
4°C
Enzyme Diluent
18 mL x 1
4°C
Enzyme
Powder x 1
-20°C, keep in dark
Standard
Powder x 1
4°C, keep in dark
Instruction Manual
1
Storage
2 - 8°C
Detection Range
50 umol/L - 500 umol/L
General Description
Dehydroascorbic acid (DHA) is an oxidized form of ascorbic acid (vitamin C). It is actively imported into the endoplasmic reticulum of cells via glucose transporters. The assay is initiated with the reduction reaction of the DHA by DTT. The reduction reaction products AsA can be measured at a colorimetric readout at 265 nm.
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