Cry1C ELISA Kit

Sample

Crop samples

Species Reactivity

Plant

Detection Method

sELISA

Intended Use

This kit employs a double‑antibody sandwich ELISA method to quantitatively detect the content of Cry1C protein in crop samples (leaves, seeds, and bulk grains).

Contents of Kit

1. Cry1C Coated Plate, 96 wells
2. Cry1C Standard, 2 vials
3. 11× Concentrated Enzyme Conjugate (anti‑Cry1C‑HRP), 1 bottle
4. Enzyme Diluent, 1 bottle
5. Chromogen (TMB Substrate), 1 bottle
6. Stop Solution (2 M H₂SO₄), 1 bottle
7. 20× Concentrated Wash Buffer, 1 bottle
8. Sample Extraction Buffer, 1 bottle

Storage

Store the kit at 2–8°C. Do not freeze. Unused strips must be resealed and stored at 2–8°C.

Standard Curve

Quantitative Analysis
Subtract the blank absorbance (average of the wells containing Sample Extraction Buffer) from the absorbance of all standards and samples.
Plot a standard curve with the blank‑corrected absorbance (OD) on the y‑axis and the Cry1C standard concentrations (ppb) on the x‑axis.
Use linear regression or a four‑parameter logistic fit to determine the concentration of each sample. Multiply by the dilution factor to obtain the Cry1C content in the original sample.