Sample
Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Copper (Cu2+) content in plasma, tissue extracts, cell lysate, cell culture media, and other biological fluid samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 4 mL x 1 | 4°C |
| Masking Reagent | Powder x 1 | 4°C |
| Dye Reagent | Powder x 1 | 4°C, keep in dark |
| Dye Reagent Diluent | 1 mL x 1 | 4°C |
| Standard ( umol/L) | 250 | 1 mL x 1 | 4°C |
| Instruction Manual | 1 | |
Detection Range
1 umol/L - 250 umol/L
General Description
Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper is mainly present in caeruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson's disease, microcytic hypochromic anemia and bone disease due to reduced collagen synthesis. Simple, direct and automation-ready procedures for measuring copper concentrations find wide applications in research, drug discovery and environmental monitoring. This assay kit utilizes a chromogen that forms a colored complex specifically with copper ions. The reaction products can be measured at a colorimetric read out at 605 nm.
Citations
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