Human CCL19 ELISA kit

Sample

serum, plasma, cell culture supernatants, urine, cell and tissue lysates

Species Reactivity

Human

Intended Use

The CCL19 (Human) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-3 beta in biological samples, such as serum, plasma, cell culture supernatants, urine, and/or cell and tissue lysates.

Contents of Kit

1. Antibody-coated ELISA Plate (Item A) - 96 wells (12 strips x 8 wells) coated with specific capture antibody.
2. 20x Wash Buffer (Item B) - RABWASH4: 25 ml of 20x concentrated solution.
3. Target Protein Standard (Item C) - 2 vials, recombinant protein.
4. Assay/Sample Diluent Buffer/s: See current Certificate of Analysis
5. Biotinylated Detection Antibody (Item F) 2 vials of biotinylated detection antibody (each vial is enough to assay half a microplate).
6. HRP-Streptavidin (Item G) - RABHRP5: 200 μl of concentrated HRP-conjugated streptavidin.
7. ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) - RABTMB3: 12 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffer solution.
8. ELISA Stop Solution (Item I) - RABSTOP3: 8 ml of 0.2 M sulfuric acid.

Storage

Store the kit at -20°C. It remains active for up to 1 year. Avoid repeated freeze-thaw cycles.
The reconstituted standard should be stored at -20°C or -70°C (-70°C is recommended). Opened microplate strips or reagents may be store for up to 1 month at 2-8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Standard Curve