Sample
Honey, milk, juice, and rice
Detection Method
Competitive-ELISA
Intended Use
This kit can be used in quantitative and qualitative analysis of carbendazim residue in honey, milk, juice, and rice.
Contents of Kit
| No. | Components | Size | Storage Conditions |
| 1 | Microtiter plate | 96T | 2-8°C |
| 2 | Standard solutions | 5 × 0.8ml | 2-8°C |
| 3 | Spiking standard solution | 0.8ml | 2-8°C |
| 4 | Carbendazim Antibody#1 | 11ml | 2-8°C |
| 5 | 100X HRP-Conjugated Antibody#2 | 0.25ml | 2-8°C |
| 6 | Antibody#2 Diluent | 20ml | 2-8°C |
| 7 | 20X Wash solution | 28ml | 2-8°C |
| 8 | TMB Substrate solution | 12ml | 2-8°C |
| 9 | Stop solution | 14ml | 2-8°C |
Storage
Storage condition: 2-8°C
Storage period: 12 months
Performance Characteristics
CV < 10%
Detection Limit
Milk/Juice/water: 0.5ppb
Honey: 1ppb
Rice/produce/processed food: 1ppb
Sensitivity
Overall Sensitivity: 0.1ppb
General Description
Carbendazim is a fungicide used for the treatment and control of fungal diseases. Carbendazim continues to be used, frequently in combination with other fungicides. The greatest use of Carbendazim occurs in Europe and Asia. It is among the twelve pesticides most frequently found in European Union (EU) monitoring programs. Carbendazim is a potential endocrine disruptor and animal studies have shown in utero exposure to cause severe physical deformities including the lack of formation of eyes and the development of hydrocephalus, or water on the brain. Studies have also shown reproductive effects including impaired testicular development and functioning and infertility. The European Commission has placed Carbendazim on a priority list of chemicals affecting the function of hormones. Carbendazim is also highly toxic to aquatic life. The current EU MRLs for Carbendazim on fresh produce vary according to item but are in the range of 0.1-0.7 mg/kg. In the United States, Carbendazim is permitted for use only in paints and adhesives, in textiles, and for ornamental trees. It is not approved for use on foods; however, Carbendazim has been found in foods in the US, including baby food in 2000 and imported orange juice in 2012. The monitoring of water sources and food products, including fresh produce and juices, is necessary to ascertain that Carbendazim is not present at levels which present a danger to human health.
This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.
Citations
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