Bilirubin Assay Kit

Sample

Serum, Urine, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of bilirubin content in serum, urine, and other biological fluid samples.

Contents of Kit

Storage

2 - 8°C

Detection Range

1 umol/L - 200 umol/L

General Description

Bilirubin is a yellow compound that occurs in the normal catabolic pathway that breaks down heme in vertebrates. This catabolism is a necessary process in the body's clearance of waste products that arise from the destruction of aged red blood cells. Bilirubin is one of the degradation products of hemoglobin formed when red blood cells die. Bilirubin exists in the insoluble unconjugated form (also indirect bilirubin), or soluble glucuronide conjugated form bilirubin (also direct bilirubin). Conjugated bilirubin moves into the bile canaliculi of the liver and then to the gall bladder. When stimulated by eating, bile (including the conjugated bilirubin) is excreted into the small intestine, where bilirubin is converted into urobilinogen. Bilirubin is a key diagnostic indicator. High levels of bilirubin result when too much hemoglobin is broken down or the removal of bilirubin does not function properly. The accumulation of bilirubin in the body causes jaundice. Bilirubin Microplate Assay Kit is designed to measure bilirubin in various samples in 96-well microplate. The vanadate oxidation method is used for testing the bilirubin. The color intensity at 630 nm is directly proportional to Bilirubin concentration in the sample.