Barbiturate ELISA Kit

Sample

Plasma, Serum, Urine, Whole Blood

Species Reactivity

Human

Detection Method

cELISA

Intended Use

The Barbiturate ELISA Kit is a specific and sensitive in-vitro test to detect the presence of barbiturates in samples such as whole blood, serum, plasma and urine. This kit has been configured for research use only and is not for diagnostic and clinical use.

Contents of Kit

1. Microwells with polyclonal anti-barbiturate: 12×8×1
2. Barbiturate-Conjugate: 12 ml
3. Immunalysis Positive Reference Standard: 2 ml
4. Negative Standard: 1 ml
5. TMB Substrate: 12 ml
6. Stop Reagent: 11 ml

Storage

1. Store the kit at 2-8°C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun, or strong light.

Precision

The precision of the Barbiturate ELISA Kit has been verified by assessment of the mean, standard deviation (SD) and coefficients of variation (CV) in data resulting from repetitive assays.
Precision Intra-Assay Precision was determined with reference controls. A 0, 5, 10 and 25 ng/ml standard was assayed eight times in the same assay.

Sensitivity

Assay sensitivity based on the minimum secobarbital concentration required to produce a four standard deviation from assay Ao is 1 ng/ml.

General Description

Barbiturates - derivatives of Barbituric acid - are sedative drugs which at low doses induce relaxation and at high doses induce coma and even death. Barbiturates are usually administered orally but may also be taken intravenously or intramuscularly and are absorbed rapidly. The metabolism of Barbiturates is mainly in the liver, a number of metabolic pathways have been described which include oxidation, desulfuration and ring cleavage. Because the number and the proportion of the various Barbiturate metabolites varies with each individual the results are expressed in terms of the equivalents of the standard, secobarbital/ml.

Standard Curve

The following data represent a typical dose/response curve.

The dose/response curve shown above should not be used in assay calculations. It is recommended that at least one in-house positive quality control sample be included with every assay run. A dose response curve or a cutoff calibrator should be run with every plate.