Atenolol ELISA Kit

Sample

Urine

Species Reactivity

Human

Detection Method

cELISA

Intended Use

Enzyme Immunoassay for the determination of atenolol in sample.

Contents of Kit

1. 96-wells microtiter plate (#S). Twelve strips of 8 detachable wells, coated with Anti-Atenolol antibody. 96 (8×12) wells
2. Calibrators containing 0, 0.5, 2 and 8 ng/mL of Atenolol. 0.6 mL × 4
3. Atenolol-Horseradish Peroxidase Conjugate (ATL-HRP) (#3). 10.5 mL
4. Stabilized tetramethylbenzidine (TMB) substrate. Ready to use. (#5) 10.5 mL
5. Wash Buffer (10xPBS-Tween). Dilute 10 fold with distilled or deionized water to 150 mL prior to use. (#6) 15 mL
6. Stop Solution, 3 N HCl. (#7) 10.5 mL

Storage

All reagents of the kit are stable, if stored at 2-8°C, until the expiration date stated on the kit.

General Description

Atenolol, 4-(2-hydroxy-3-isopropyl-aminopropoxy)-phenylacetamide, is a β1 selective adrenoceptor antagonist used in the treatment of angina and hypertension. Atenolol is poorly protein bound. Plasma half-life is 6-8 hours but can rise to very long hours in severe renal impairment. There is no liver metabolism, 90% of absorbed drug is excreted unchanged in the urine.

Standard Curve