Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Aspartate Transaminase (AST) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Substrate | Powder x 1 | 4°C, keep in dark |
| Substrate Diluent | 5 mL x 1 | 4°C |
| Dye Reagent I | 5 mL x 1 | 4°C |
| Dye Reagent II | 10 mL x 1 | 4°C |
| Standard | Powder x 1 | 4°C |
| Positive Control | Powder x 1 | -20°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.625 umol/mL - 20 umol/mL
General Description
Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, and trauma. The enzyme catalyzed reaction product phenylhydrazone can be measured at a colorimetric readout at 520 nm.
Citations
Publication ()
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