Cell membranes from the TG115 human liver tumor cell line
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0
Clone
GI6
Cell Line Description
Animals were immunized with cell membranes from the TG115 human liver tumor cell line. Spleen cells were fused with Sp2/0 myeloma cells. The antibody is specific for trifucosylated type 2 glycolipids, but cross-reacts somewhat with difucosylated type 2 chains. It does not react with monofucosylated type 2 chains.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 5x10^4 cells/mL and maintain cultures between 1x10^5-2x10^6 cells/ml. The cell line is best carried as an ascites in pristane primed BALB/c mice
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
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