A peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
FOX-NY
Clone
8C5
Cell Line Description
Animals were immunized with a peptide synthesized to correspond to amino acids 84 to 104 of the predicted protein that occur between the RNA recognition motif and the SR domain. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with a single member, SRp20, of the family of essential splicing factors the SR proteins.
Application Notes
To determine if a factor in RNA metabolism is SRp20. IP, WB and ICC assays
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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