Animals were immunized with purified reovirus type 3 virions. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody neutralizes reovirus type 3 and inhibits the hemagglutinating activity of the virus. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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