High molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse
Clone
31I22
Cell Line Description
Peripheral blood B cells from a patient immunized with high molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus. The antibody reacts with P aeruginosa Fisher immunotypes 2, 6 and 7. The antibody cross-reacts extensively with clinical isolates of P. aeruginosa bearng type b flagella.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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