Toxoid preparation purified from the NOR-1 strain of P. multocida
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
2C3B4
Cell Line Description
Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida. The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990.
Application Notes
It can be used to identify toxigenic strains of P. multocida
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
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