Animals were immunized with C57BL/6 mouse liver derived large granular lymphocytes in the presence of rat antiserum against NK cell depleted C57BL/6 mouse spleen cells. Spleen cells were fused with NS-1 myeloma cells. The antibody specially reacts with a major (approximately 50%) subset of mouse NK cells. This subset (LGL-1+/NK-1.1+) functions in NK type lysis, but lacks LAK (lymphokine activated killing) activity against P815 and L5178Y tumor target cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
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