Mouse Anti-Human ITGB3 Hybridoma [BQ-4]

Immunogen

Partially purified human platelet glycoproteins IIb and IIIa (GPIIb and GPIIIa, respectively)

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

NS-1

Clone

BQ-4

Cell Line Description

Animals were immunized with partially purified human platelet glycoproteins IIb and IIIa (GPIIb and GPIIIa, respectively). Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes all known allelic forms of the beta 3 subunit of the integrin superfamily. The two glycoproteins (GPIIb and GPIIIa) together form the platelet binding site for fibrinogen. The antibody reacts specifically with GPIIIa, but does not inhibit platelet aggregation. It is useful for studies of human GPIIIa expression by flow cytometry, Western blot (non-reduced) and affinity chromatography methods. The antibody can also be used for the classification of human megakaryocytic leukemias and Glanzmann thrombasthenia.

Application Notes

Studies of human GPIIIa expression by FC, WB (non-reduced) and affinity chromatography methods.
The classification of human megakaryocytic leukemias and Glanzmann thrombasthenia.

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 10%(v/v) DMSO

Synonyms

ITGB3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); GP3A; integrin beta-3; CD61; GPIIIa; platelet glycoprotein IIIa; platelet membrane glycoprotein IIIa; GT; BDPLT2;

Entrez Gene ID

UniProt ID