IL-1 activated human umbilical vein endothelial cells
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Clone
8B0
Cell Line Description
Animals were immunized with IL-1 activated human umbilical vein endothelial cells. Spleen cells were fused with mouse myeloma cells. The antibody binds to IL-1 activated human endothelial cells, but not to resting endothelial cells or IL-1 treated mononuclear leukocytes, fibroblasts or keratinocytes. It precipitates an antigen consisting of a 100000 dalton polypeptide (90000 daltons under reducing conditions). The antibody totally blocks binding of granulocytes and partially blocks monocyte binding to IL-1 stimulated endothelial cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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