Mouse Anti-Human IgM, lambda Hybridoma [MQ5.5]

Immunogen

Human IgM, kappa paraprotein (LP) which reacted with intermediate filaments

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse Lymph node

Myeloma

P3X63Ag8.653

Clone

MQ5.5

Cell Line Description

Animals were immunized with a human IgM, kappa paraprotein (LP) which reacted with intermediate filaments. Lymph node cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with most human IgM, lambda and some human IgM, kappa molecules. It appears to react with a conformational determinant expressed preferentially on IgM, lambda complexes. It also reacts with mu - surrogate light chain complexes, and provides a marker for pre-B and early B cell differentiation. It does not react with isolated mu, lambda or kappa chains.

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.