Animals were immunized with a human IgE paraprotein (PS). Lymph node cells were fused with S194/5.XXO.BU.1 myeloma cells. The antibody reacts with human epsilon heavy chain. It has a very high affinity (9 X 10^-10 m/L) for an epitope located between amino acids 218 and 301 (CH2 domain) of human epsilon. It does not bind to protein A, but functions well in histamine release assays.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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