Anti-HRP monoclonal antibody, clone 4J23

In this work, the peroxidase-like properties of dendritic Au-Pt nanoparticles (30 similar to 60 nm) were investigated and these nanoparticles were applied in the immunoassay as signal labels for the detection of capsular polysaccharides (CPS, similar to 300 kDa, expressed on the cell envelopes ofBurkholderia pseudomalleicausing melioidosis). The immunoassay adopts monoclonal anti-CPS antibodies. The assay limit of detection (LOD), which is calculated through the 3 sigma method, is at 0.36 ng/mL for CPS spiked in PBS with 5% milk, and at 0.88 ng/mL for CPS spiked in human serum. The results from this work show that the dendritic Au-Pt nanoparticles can produce similar assay performance in detecting CPS as HRP did. Considering these particles can be easily prepared and stable in environments (thus avoiding cold-chain based storage/transportation), they have potential to be applicable for both in-house and in-field immunoassay development for the pathogenB. pseudomalleidetection.

Considering the threat of H2O2 to human health and environmental security, it is of great significance to develop rapid, accurate and sensitive analytical methods for H2O2 detection. In this work, porous regular hexagonalshaped FeS2 nanosheets (NSs) with a side length of about 1 mu m are employed as a peroxidase mimic to detect H2O2. The Km of the FeS2 NSs for H2O2 (0.00342 mM) is much lower than actual enzyme HRP (3.7 mM). The FeS2 NSs exhibit high sensitivity in linear range of 0.02-4.00 mu M of H2O2 with a limit of detection (LOD) of 7.60 nM (S/N = 3). The Fe ion in the FeS2 NSs is the active site. No obvious effect on the determination was found when K+, NH4+, Ni2+, Mn2+, Cu2+, Zn2+, Al3+, Ba2+, Ca2+, NO3-, glucose and sucrose were added in the solution, respectively. The FeS2 NSs were also applied to rapidly detect H2O2 concentrations in different actual samples, such as lens solution, beer and disinfectant.