C57BL/10 mouse spleen cells enriched for T lymphocytes
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Myeloma
NS-1
Clone
N2/70.27.22.IM
Cell Line Description
Animals immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an antigen present on mouse red blood cells. Thymocytes, mature B lymphocytes, granulocytes and monocytes but not peripheral T lymphocytes. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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