Animals were immunized with CMV infected human fibroblasts. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a 72000 dalton HMCV immediate - early perinuclear antigen. The antigen is expressed within three hours of infection with HCMV.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
Have you cited CSC-H0977 in a publication? Let us know and earn a reward for your research.
My Review for Mouse Anti-Human HCMV Hybridoma [M-25]
Creative Diagnostics products are for RESEARCH USE ONLY, please make sure your review is research based.
Required fields are marked with *
Terms and conditions:
We will select high-quality review customers and offer a $30 coupon for your next purchase.
All product reviews must be submitted in the English language.
Creative Diagnostics will not share any personal information of applicants, and all information will be treated with strict confidentiality and will not be sold or disclosed to a third party.
COA
Publication 
Loading ......
