Animals were immunized with neuroblastoma cells (LAN-1). Spleen cells were fused with P3X63Ag8 myeloma cells. Ganglioside GD2 is strongly expressed on cells of tumors of neuroectodermal origin (especially neuroblastoma and melanoma cells). The antibody reacts strongly with neuroblastoma and melanoma cells, but does not react with lymphomas, sarcomas and most carcinomas. Faint reactivity was reported for small cell carcinomas, adenocarcinomas of the adrenal cortex, gliomas and normal neural and muscle tissue.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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