Mouse Anti Drosophila spectrin, Alpha Hybridoma [4B0 (434 or N21-3)]

The dominant paradigm for spectrin function is that (alpha beta)(2)-spectrin tetramers or higher order oligomers form membrane-associated two-dimensional networks in association with F-actin to reinforce the plasma membrane. Tetramerization is an essential event in such structures. We characterize the tetramerization interaction between alpha-spectrin and beta-spectrins in Drosophila. Wild-type alpha-spectrin binds to both beta- and beta(H)-chains with high affinity, resembling other non-erythroid spectrins. However, alpha-spec(R22S), a tetramerization site mutant homologous to the pathological alpha-spec(R28S) allele in humans, eliminates detectable binding to alpha-spectrin and reduces binding to beta(H)-spectrin similar to 1000-fold. Even though spectrins are essential proteins, alpha-spectrin(R22S) rescues alpha-spectrin mutants to adulthood with only minor phenotypes indicating that tetramerization, and thus conventional network formation, is not the essential function of non-erythroid spectrin. Our data provide the first rigorous test for the general requirement for tetramer-based non-erythroid spectrin networks throughout an organism and find that they have very limited roles, in direct contrast to the current paradigm.

In this paper we aim to validate a methodology designed to obtain H alpha emission line fluxes from J-PLUS photometric data. J-PLUS is a multi narrow-band filter survey carried out with the 2 deg(2) field of view T80Cam camera, mounted on the JAST/T80 telescope in the OAJ, Teruel, Spain. The information of the twelve J-PLUS bands, including the J0660 narrow-band filter located at rest-frame H alpha, is used over the first 42 deg(2) observed to retrieve de-reddened and [NII] decontaminated H alpha emission line fluxes of 46 star-forming regions with previous SDSS and/or CALIFA spectroscopic information. The agreement between the J-PLUS H alpha fluxes and those obtained with spectroscopic data is remarkable, finding a median comparison ratio with a scatter of R = F-H alpha(J-PLUS)/F-H alpha(spec) = 1.05 +/- 0.25. This demonstrates that it is possible to retrieve reliable H alpha emission line fluxes from J-PLUS photometric data. With an expected area of thousands of square degrees upon completion, the J-PLUS dataset will allow the study of several star formation science cases in the nearby universe, as the spatially resolved star formation rate of nearby galaxies at z <= 0.015, and how it is influenced by the environment, morphology, stellar mass, and nuclear activity. As an illustrative example, the close pair of interacting galaxies NGC 3994 and NGC 3995 is analysed, finding an enhancement of the star formation rate not only in the centre, but also in outer parts of the disk of NGC 3994.