Mouse Anti Drosophila Groucho protein Hybridoma [anti-Gro]

Regulation of miR-146a abundance and its role in intestinal inflammation and particularly in intestinal epithelial cells (IECs) has been poorly studied. Here we study the relationship between bacterial antigens and inflammatory stimuli, and miR-146a expression using IEC lines and models of colitis (trinitrobenzenesulfonic acid (TNBS), dextran sulfate sodium (DSS) and the CD4 + CD62L + T cell transfer model). Specific bacterial antigens and cytokines (LPS, flagelin and IL-1 beta/TNF) stimulate miR-146a expression, while peptidoglycan, muramyldipeptide and CpG DNA have no effect. Overexpression of miR-146a by LPS depends on the activation of the TLR4/MyD88/NF-kB and Akt pathways. Accordingly, the induction of miR-146a is lower in TLR4, but not in TLR2 knock out mice in both basal and colitic conditions. miR-146a overexpression in IECs induces immune tolerance, inhibiting cytokine production (MCP-1 and GRO alpha/IL-8) in response to LPS (IEC18) or IL-1 beta (Caco-2). Intestinal inflammation induced by chemical damage to the epithelium (DSS and TNBS models) induces miR-146a, but no effect is observed in the lymphocyte transfer model. Finally, we found that miR-146a expression is upregulated in purified IECs from villi vs. crypts. Our results indicate that miR-146a is a key molecule in the interaction among IECs, inflammatory stimuli and the microbiota.

Sensitive equipment such as electric vehicle control systems and vehicle electronics are susceptible to the external electromagnetic environment. The radiated electromagnetic signals interfere with the normal driving of electric vehicle and threaten human life. In order to ensure safety and reliability of electric vehicles, electromagnetic (EM) absorbent has become a key technology. RGO/Ni was prepared by in-situ reduction method in this paper. Since composition, size, and special morphology have a vital influence on microwave absorption properties, the as-obtained products were characterized by X-ray diffraction, scanning electron microscopy and vector network analysis. Different reduction methods will bring different dielectric properties to the obtained RGO. The RGO obtained by Zn powder have a more complete structure, and therefore better dielectric properties. The prepared RGO/Ni-2 composite possesses excellent impedance matching characteristics and electromagnetic complementary effect, which are beneficial to microwave absorption performance. In this paper, the absorption mechanism of RGO/Ni-2 is studied, which provides an effective way for the preparation of GRO/Ni, and introduces the application of RGO/Ni as a high-efficiency absorber in the field of electric vehicles.