DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. In tissue sections, MG38 identifies DEC-205 on thymic cortical epithelium and dendritic cells in the thymic medulla and tonsillar T cell areas. The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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