Liposomes containing high amounts of cholesterol and lipid A as an adjuvant
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8
Clone
3D6-7
Cell Line Description
Animals were immunized with liposomes containing high amounts of cholesterol and lipid A as an adjuvant. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody specially reacts with cholesterol in liposomes containing 71% cholesterol but not with liposomes containing 43% cholesterol. It reacts with crystalline cholesterol in a solid-phase enzyme-linked immunosorbent assay. The antibody is capable of detecting cholesterol in situ in the membranes of skeletal muscle sections.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 2x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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