Purified CD34 positive human progenitor stem cells
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse Lymph node
Myeloma
Sp2/0 Ag14
Clone
BD244.2
Cell Line Description
Animals were immunized with purified CD34 positive human progenitor stem cells, which had been pre-incubated with phytohemagglutinin (PHA). Lymph node cells were fused with Sp2/0 Ag14 mouse myeloma cells. The antibody reacts with a subset of hematopoietic progenitor cells derived from human bone marrow, fetal bone marrow and liver, cord blood and adult peripheral blood. The subset of cells recognized by AC133 is CD34bright, and contains substantially all of the CFU-GM activity present in the CD34+ population.
Application Notes
A reagent for isolating and characterizing human hematopoietic progenitor and stem cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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