Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the murine T cell receptor (CD3 - T3) complex. The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor. It reacts with all mature T cells and can both activate and inhibit T cell function. The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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