Animals were immunized with human epidermal cells. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody binds to a cell surface antigen present on and characteristic of human basal cells and malignant squamous cells. The antibody does not react with normal human fibroblasts, endothelial or hematopoietic cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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