Mouse Anti-Human Astrocyte Protein Hybridoma [K2-42]

Immunogen

Human cerebral white matter from a multiple sclerosis (MS) patient

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

NS-1

Clone

K2-42

Cell Line Description

Animals were immunized with human cerebral white matter from a multiple sclerosis (MS) patient. Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes an intracellular protein antigen (MW 30000) expressed by human and rat astrocytes and other specialized glia. (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS). This marker is distinct from glial fibrillary acidic protein (GFAP) and vimentin. Astrocytes in the vicinity of a CNS lesion show enhanced staining for J1-31 antigen. J1-31 monoclonal antibody recognizes a protein whose expression is increased following CNS injury. The antibody is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures.

Application Notes

The antibody is IF, ICC, and WBting procedures.

Morphology

Lymphoblast

Growth Properties

Suspension

Storage

Liquid nitrogen vapor phase.




Freezing medium: to complete growth medium, add 5%(v/v) DMSO