Phospho Acetyl-CoA Carboxylase Ser79 ELISA Kit

Sample

cell lysates

Species Reactivity

Human

Intended Use

The Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetyl-CoA carboxylase (ACC) when phosphorylated at Ser79.

Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit detects endogenous levels of ACC protein when phosphorylated at Ser79 as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Figure 1. Treatment of Hep G2 cells with H2 O2 stimulates phosphorylation of ACC at Ser79, detected by the PhosphoACC (Ser79) Sandwich ELISA Kit, but does not affect the levels of total ACC detected by Total ACC Sandwich ELISA Kit. Hep G2 cells (80-90% confluent) were treated 10 mM hydrogen peroxide for 10 minutes and lysed with. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Acetyl-CoA Carboxylase Rabbit mAb (left panel) or Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody (right panel) are shown in the bottom figure.

Figure 2. The relationship between the protein concentration of lysates from untreated and H2O2-treated Hep G2 cells and the absorbance at 450 nm using the Phospho-Acetyl-CoA Carboxylase (Ser79) Sandwich ELISA Kit is shown.

General Description

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).