Mouse anti-Human PGM1 monoclonal antibody, clone 4C9I5

The physiology and genetics underlying juvenility is poorly understood. Here, we exploit Arabidopsis as a system to understand the mechanisms that regulate floral incompetence during juvenility. Using an experimental assay that allows the length of juvenility to be estimated and mutants impaired in different pathways, we show that multiple inputs influence juvenility. Juvenile phase lengths of wild type (WT) accessions Col-0, Ler-0 and Ws-4 are shown to differ, with Col-0 having the shortest and Ws-4 the longest length. Plants defective in sugar signalling [gin1-1, gin2-1, gin6 (abi4)] and floral repressor mutants [hst1, tfl1, tfl2 (lhp1)] showed shortened juvenile phase lengths compared to their respective WTs. Mutants defective in starch anabolism (adg1-1, pgm1) and catabolism (sex1, sex4, bam3) showed prolonged juvenile phase lengths compared to Col-0. Examination of diurnal metabolite changes in adg1-1 and sex1 mutants indicates that their altered juvenile phase length may be due to lack of starch turnover, which influences carbohydrate availability. In this article, we propose a model in which a variety of signals including floral activators and repressors modulate the juvenile-to-adult phase transition. The role of carbohydrates may be in their capacity as nutrients, osmotic regulators, signalling molecules and/ or through their interaction with phytohormonal networks.

Linkage tests were conducted between an esterase locus, Est1, and loci coding for four isozymes and two morphological markers in a cross between cultivars Shiratsurunoko and Suzuhime, The loci tested were Dial(diaphorase), Enp (endopeptidase), Idh2 (isocitrate dehydrogenase), Pgm1 (phosphoglucomutase), Ln (leaf shape), and W1 (flower or hypocotyl color). A contingency chi-square test indicat ed a significant deviation from the expectation of independent inheritance between the Est1 and the Enp and Ln loci, members of linkage group 4. Based on the combined F-2 and F-3 data, recombination values were estimated as 32.3 +/- 2.1% for Est1 versus Enp and 20.4 +/- 2.2% for Est1 versus Ln. The recombination value between the Enp and Ln loci was calculated as 15.8 +/- 2.0%. The Est1 locus was therefore assigned to linkage group 4, and the gene gene order was determined as Est1-Ln-Enp.