Human soluble Nectin-4 ELISA Kit

Sample

cell culture supernatant

Species Reactivity

Human

Intended Use

The Human soluble Nectin-4 ELISA Kit is used for the quantitative measurement of the soluble form of human nectin-4 in cell culture supernatant.

Storage

- Upon receipt store all components at 4°C.
- Don't expose reagents to excessive light.

Precision

Intra-assay (Within-Run, n=16) CV=3.7, 3.0, 3.8 %
Inter-assay (Run-to-Run, n=5) CV=6.5, 4.2, 6.5 %

Sensitivity

The limit of detection (defined as such a concentration of human soluble nectin-4 giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 5.8 pg/mL of sample.

General Description

Nectins are a family of type I transmembrane proteins that are involved in a Ca2+-independent immunoglobulin-like cell-cell adhesion molecules consisting of four members, Nectin-1-4. Nectin-4, also known as PVRL4, LNIR or PRR4, is expressed in the embryo and the placenta, whereas the other nectins are expressed widely in human adult tissues. The extracellular domain of nectin-4 is cleaved at the cell surface via the proteolytic activity of ADAM17/TACE, and the soluble form is released into the blood. Increased levels of the circulating form of nectin-4 have been detected in the sera of patients with metastatic breast cancer. The soluble form of nectin-4 has also been detected in significant amounts in the sera of patients with non-small cell lung cancer (NSCLC), and the up-regulation of soluble nectin-4 in the serum has been suggested to be associated with a poor prognosis for NSCLC.
Nectin-4 is overexpressed in a variety of cancers other than the above and is currently under clinical investigation as a therapeutic target. Oncolytic virotherapy is a novel treatment strategy for cancer. Vaccine and laboratory-adapted strains of measles virus (MV) uses nectin-4 and CD46 molecule as major entry receptors into cells. A recombinant measles virus which selectively uses nectin-4 receptor is used in oncolytic agents for cancer. Also anti-nectin-4 antibodies such as enfortumab (AGS-22M6E) are under clinical investigation for cancer therapies. Furthermore mutations in nectin-4 are associated with ectodermal dysplasia-syndactyly syndrome 1 (EDSS1).