One unit is defined as the amount of enzyme which converts 1 μmole of fructose‒6‒phosphate to Fructose‒1,6 ‒ bisphosphate per minute at 37°C under the conditions specified in the assay procedure.
Target
Phosphofructokinase
Molecular Weight
35 kDa
Application Notes
This enzyme is useful for enzymatic determiantion of fructose-6-phosphate
Format
Lyophilized
Buffer
Enzyme dilution buffer
Storage
Store at -20°C
Introduction
The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes. Phosphofructokinase catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate, a key regulatory step in the glycolytic pathway. It is allosterically inhibited by ATP and allosterically activated by AMP, thus indicating the cell's energetic needs when it undergoes the glycolytic pathway.
Keywords
Phosphofructokinase; PFKL; PFK
Citations
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