Specifications
Immunogen
Myc tag fusion protein
Applications
Application Notes
WB 1:5,000-1:20,000; ELISA 1:5,000-1:20,000; ICC/IF 1:200-1:1000; IP 1-2ug/sample; Flow Cyt 1μg for 10^6 cells
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
General Notes
Anti-Myc tag, HiResNb™ VHH-Mouse IgG1 Fc Antibody is designed for detecting Myc tag fusion protein specifically. Anti-Myc tag, HiResNb™ VHH-Mouse IgG1 Fc Antibody is recombinant fab of alpaca IgG1 fused to mouse IgG1 Fc. Based on western blot and ELISA, Anti-Myc tag, HiResNb™ VHH-Mouse IgG1 Fc Antibody reacts with the Myc tag sequence(EQKLISEEDL) selectively, no reactivity with other proteins.
Target
Alternative Names
c Myc; c Myc Epitope Tag; c Myc Tag; Myc Epitope Tag; Myc proto-oncogene protein; Transcription factor p64;
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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Citations
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Timmers, KI; Clark, AE; et al. Identification of SNAP receptors in rat adipose cell membrane fractions and in SNARE complexes co-immunoprecipitated with epitope-tagged N-ethylmaleimide-sensitive fusion protein. BIOCHEMICAL JOURNAL 320:429-436(1996).
Teterina, NL; Pinto, Y; et al. Analysis of Poliovirus Protein 3A Interactions with Viral and Cellular Proteins in Infected Cells. JOURNAL OF VIROLOGY 85:4284-4296(2011).