Moxidectin ELISA Kit

Sample

feed, honey, kidney, liver, meat (beef, chicken and pork), milk, milk powder ,serum and urine

Detection Method

cELISA

Intended Use

The Moxidectin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Moxidectin in feed, honey, kidney, liver, meat (beef, chicken and pork), milk, milk powder ,serum and urine.

Contents of Kit

1. Moxidectin-coated Microtiter Plate, 1 × 96-well plate, (8 wells × 12 strips)
2. Moxidectin Standards:
Negative control (white cap tube) 1.0 mL
0.05 ng/mL (yellow cap tube) 1.0 mL
0.15ng/mL (orange cap tube) 1.0 mL
0.45ng/mL (pink cap tube) 1.0 mL
1.35 ng/mL (purple cap tube) 1.0 mL
4.05 ng/mL (blue cap tube) 1.0 mL
1000 ng/mL (spiking, red cap tube) 1.0 mL
3. Moxidectin Antibody #1, 6 mL
4. HRP-Conjugated Antibody #2, 6mL
5. 10× Sample Extraction Buffer, 30 mL
6. 10× Sample Dilution Buffer, 25 mL
7. 20× Wash Solution, 30 mL
8. TMB Substrate, 12 mL
9. Stop Buffer, 12 mL

Storage

Moxidectin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
*If you are not planning to use the kit for over 3 months, store Moxidectin Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Sensitivity

Detection Limit (ng/g or ppb)
Feed: 2.5
Honey: 1
Meat/Liver/Kidney: 2.5
Milk: 0.5
Milk Powder: 0.5
Serum/Urine: 0.25

Standard Curve

The following figure is a typical Moxidectin standard curve