Mouse ANCA Screen ELISA Kit

Sample

Serum, plasma

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

The Mouse ANCA Screen ELISA Kit is designed for the qualitative analysis of PR3 and MPO in Mouse Serum, plasma samples.

Contents of Kit

1. SORB MT 1 divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
2. CONTROL A – C 3x 1,5 ml Control A (negative), B (cut-off) and C (positive), containing PR3, MPO antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.009 %). Ready to use.
3. SAM DIL 5x 20 ml Sample Buffer, containing PBS, BSA, detergent, preservative NaN3 0.09 %, 5x conc.
4. ENZ CONJ 15 ml Enzyme Conjugate containing anti-mouse IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative ProClin 300 0.05 %. Ready to use.
5. SUB TMB 15 ml TMB Substrate; containing 3,3', 5,5'- Tetramethylbenzidin. Ready to use.
6. STOP SOLN 15 ml Stop solution; contains acid. Ready to use.
7. WASH SOLN 50x 20 ml Wash Buffer, containing Tris, detergent, preservative NaN3 0.09 %; 50x conc

Storage

2–8 °C, protected from light and moisture.

Detection Range

0 - 200 U

General Description

Anti-neutrophil cytoplasmic antibodies (ANCA) against proteinase 3 (PR3) or myeloperoxidase (MPO) are diagnostic markers for ANCA-associated vasculitides. PR3 is the landmark antigen in granulomatosis with polyangiitis (GPA, formerly Wegener's granulomatosis). MPO differentiates microscopic polyangiitis (MPA) and eosinophilic granulomatosis with polyangiitis (EGPA).