Mitochondrial (AMA)-M2-Ab ELISA

Sample

serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Enzyme immunoassay for the quantitative and qualitative detection of antibodies against M2 in human serum.

Storage

Store all reagents and the microplate at 2-8°C/35-46°F, in their original containers. Once prepared, reconstituted solutions are stable at 2-8°C/35-46°F for 1 month. Reagents and the microplate shall be used within the expiry date indicated on each component, only. Avoid intense exposure of TMB solution to light. Store microplates in designated foil, including the desiccant, and seal tightly.

Precision

To determine the precision of the assay, the variability (intra and inter-assay) was assessed by examining its reproducibility on three serum samples selected to represent a range over the standard curve.

Detection Range

0-300 U/ml

Sensitivity

1.0 U/ml
Testing sample buffer 30 times on Mitochondrial (AMA)-M2-Ab ELISA gave an analytical sensitivity of 1.0 U/ml.

General Description

The Mitochondrial (AMA)-M2-Ab ELISA is a solid phase enzyme immunoassay employing native mitochondial M2 antigen for the quantitative and qualitative detection of IgG antibodies against M2 in human serum.
The assay is a tool in the research of the primary biliary cirrhosis (PBC).
Primary biliary cirrhosis (PBC) is a chronic inflammatory disorder of the small and medium bile ducts and serologically characterized by the presence of circulating M2 autoantibodies. Anti-M2 autoantibodies belong to the group of anti-mitochondrial antibodies (AMA). The heterogeneously reacting specific AMA of the M2 subtype are directed against three related proteins of the α-keto acid dehydrogenase complex, which is located at the inner mitochondrial membrane. The major epitope recognized is located on the E2 subunit and the protein X of the pyruvate dehydrogenase complex (PDC). Additionally, AMA-M2-G autoantibodies recognize the (E1α and E1β) subunits of the same complex and the E2 subunit of several other multienzyme complexes, such as the 2-oxo-glutarate dehydrogenase complex (OGDC) and the branched chain 2-oxo acid dehydrogenase complex (BCOADC). The determination of AMA-M2-G is a powerful tool in diagnosing PBC.