Marburg Virus Nucleoprotein (NP) Human IgM ELISA Kit

Sample

Human serum or plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Human IgM ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgM (IgM; kit specific) antibody against pathogen antigen(s) in human serum or plasma. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.

Contents of Kit

1. Pathogen antigen coated 96-well Microplate, 96 wells (12 strips x 8 wells) coated with pathogen antigen. month at 4°C*
2. Wash Buffer Concentrate (20×) (Item B), 25 ml of 20× concentrated solution. 1 month at 4°C
3. Positive Control (Item C), 1 vial of Positive Control sample containing anti-pathogen antigen antibody. 1 week at -80°C.
4. Biotinylated Anti-Human IgM (Item F), 1 vial of solution. 5 days at 4°C
5. Biotinylated Anti-Positive Control (Item F-2), 1 vial of solution. 5 days at 4°C
6. HRP-Streptavidin concentrate (Item G), 1 vial of solution. Do not store and reuse
7. TMB One-Step Substrate Reagent (Item H), 12 ml of 3,3,5,5'-tetramethylbenzidine (TMB buffer solution). 1 month at 4°C
8. Stop Solution (Item I), 8 ml of 0.2 M sulfuric acid. N/A
9. Assay Diluent B (Item E), 15 ml of 5× concentrated buffer. 1 month at 4°C
10. 5× Sample Diluent (Item J), 25 ml of 5× diluent buffer, 0.5% proclin 300 as preservative. 1 month at 4°C
*Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
^Components in final, validated assay may differ from those of preliminary manual. Please consult final manual prior to beginning experiment.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.